The effect of treadmill running with consumption of vitamin D3 on NGF levels in Parkinsonian rat's striatum

In this study, we investigate the effect of treadmill running with consumption of 1.25 dihydroxyvitamin D3 on NGF levels in Parkinsonian rats’ striatum induced by 6-hydroxy-dopamine. 48 rats (weight 276± 10g and age 12 week) were divided into 6 groups: control, sham¡ control Parkinson, exercise Parkinson, vitamin D3 Parkinson, vitamin D3 exercise Parkinson. Exercise group: five days per week for 4 weeks during the light cycle. Rats were run twice per day at a speed of 15 m/min separated by at least 1 hr; each running bout lasted 15 min for a total of 30 min per day. Parkinson supplement group in during study period received 1 µg/ml/kg VD3 intraperitoneally Two times per week. To induce Parkinson, 250 mg/kg in volume 5m/l of 6-hydroxydopamine (6-OHDA) was administered into striatum by a stereotaxic apparatus. NGF level in the striatum were measured by ELISA. Alone-intensity exercise the protocol presented in this study, nerve growth factor levels Increased but not significant(P=0.372). On the other, the supplementation VD3 alone levels of nerve growth factor (P=0.002) to increase significantly¡ also combining it with exercise could NGF levels (P=0.0001) shows a significant increase. Our findings show that pre-treatment with treadmill running and consumption supplement of 1.25 dihydroxyvitamin D3 have a protective role against 6-OHDA induced toxicity.