Effect of nitrogen fertilizer and Pseudomonas putida inoculation on yield and yield component of soybean as second crop in Moghan region

ABSTRACT Application of plant growth promoting rhizobacteria not only increases plant growth and seed yield but reduces chemical fertilizers rate and increases the efficiency of fertilizer consumption, too. To evaluate the effect of Pseudomonas putida inoculum under different levels of nitrogen fertilizer a factorial experiment based on randomized complete block design with three replications was conducted on soybean as second crop in Bilehsavar Moghan region. Experimental factors consisted of inoculated and non-inoculated seeds with P. putida, and three levels of nitrogen fertilizer (0, 50 and 100 kg N/ha as urea). The interaction of bacteria and nitrogen fertilizer on pod and grain per plant, branch number and grain yield was significant. Inoculation with bacteria and application of fertilizers increased the number of pods and seeds per plant, number of branches and seed yield, while plant height were significantly decreased in inoculated treatments increasing nitrogen level from 50 to 100. Also, nitrogen fertilizer application increased and decreased thousand seed weight in inoculated and un-inoculated treatments, respectively. The highest grain yield 3006 kg/ha obtained in 100 kg/ha nitrogen application with inoculation with bacteria. On the whole, in soybean second crop, application of 100 kg N/ha under P. putida inoculation is recommending.