Isolation and Cloning of Isoflavone Synthase Gene from Soybean (Glycin max) Williams III Cultivar
Author(s):
Abstract:
Isoflavonoids are a group of flavonoid compound which produced from flavanon precursors in legums¡ by isoflavone synthase (IFS) enzyme. The most important isoflavonoids finding in soybean are genistein and daidzein that have many medicinal impacts. Investigations have revealed that regularly using these compounds lowered the risk to come down with hormone-related cancers¡ besides menopausal symptoms and reduction in osteoprosis¡ balance in cholesterol bloods level¡ improving in heart-coronary diseases are benefits that are related to using these compounds. In this investigation in order to isolate isoflavone synthase¡ at first total RNA was extracted and cDNA was synthesized¡ subsequently. IFS gene was isolated and subjected to PCR by specific primers which have the HindIII and XbaI at their 5′ ends. Subsequently¡ amplified gene after extracting on agarose gel was cloned in restriction site of XbaI/HnidIII of pET28a. At the last step¡ recombinant plasmids were approved by PCR and enzymatic digestion. Finally¡ the mentioned vector can be used either as an expression vector in prokaryotic hosts or introduced into plants by agrobacterium or gene gun-mediated transformations.
Keywords:
Language:
Persian
Published:
Journal of Crop Breeding, Volume:8 Issue: 17, 2016
Pages:
160 to 165
magiran.com/p1590334
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!