Molecular detection of Campylobacter rectus in periodontal lesions of the patients referred to dental clinic of Tehran university

Campylobacter rectus is one of the important periodontopathogenic bacteria in many regions of the world. According to lack of published surveys on the bacterium in Iran, the aim of this research was to detect of Campylobacter rectus in the patients with periodontitis referred to dental clinic of Tehran University using molecular method.
Sampling from 40 patients was performed by clinic specialists at deepest site of periodontal pockets using paper points and corresponding data were registered. DNA of the specimens were extracted using DNP TM kit (Cinnagen) and PCR was carried out using C. rectus specific primers and ATCC 32238 reference strain as positive control. Statistically significant differences in frequency of bacterial contamination and mean depth of gingival sulcus among different age and sex groups were evaluated using SPSS 22 (Chi square and t-tests).
Molecular method results showed the amplification of 598bp long segment and C. rectus contamination in 18 (45%) of tested samples. Mean depth of gingival sulcus in women was more but the differences in frequency of bacterial contamination between men and women and mean depth of gingival sulcus among different age groups were not statistically significant.
The finding of this survey confirmed the presence of C. rectus in remarkable percentage of the periodontitis cases and pointed to the suitability of the PCR as an easy and rapid method for detection of this bacterium in clinical samples.