Effect of Zinc Oxide Nanoparticles on Inflammatory Factor TNF- β, Value of serum LDH and Tissue changes in the Mice Liver

Following this study, the effect of zinc oxide nanoparticles on the liver histopathological features and TGF-β and LDH enzyme activity was studied. Ninety male mice were randomly allocated into three groups each whit three repeat in each groups. Group 1and 2 received Zn oxide nanoparticles orally and intra-peritoneal respectively at dose of 2.5 mg/kg for 14 consecutive days; group 3 served as the control. The value of TGF-β and level of LDH enzyme were determined on 14 day and liver samples were collected at supplementation intervals, 14 day. Results showed that TGF-β value in groups 1 and 2 were higher on day 14 compared to the controls, but this increase was no significant (P>0.05). It was also found that on day 14, the activity of LDH enzyme in both treated groups were significantly higher than those of the controls (P0.05). The histopathological results showed that the alterations in the hepatocytes were mainly nuclear enlargement, as nucleus was observed in large and small sizes. Some of the Kupffer cells into hepatic sinusoids included a large nucleus. Hyperemia in central veins was evident and RBCs had infiltrated into matrix. In intra-peritoneal group cytoplasm of hepatocytes showed vacuolization. According to the results it can be concluded that zinc oxide nanoparticles cause changes in the structure of the liver and liver enzymes.