Association between polymorphism in myostatin gene (MSTN) with quantitative traits in domesticated rainbow trout (Oncorhynchus mykiss) using PCR-SSCP method
Author(s):
Abstract:
In this study the rainbow trout (Oncorhynchus mykiss) myostatin gene (MSTN) for detecting gene derived SNPs and performing genetic association study between MSTN polymorphisms and growth traits were analyzed. Twenty broodstocks of rainbow trout in Genetic and Breeding for coldwater Fishes center in Yasouj for generating full sibling were selected and tagged and then juveniles were raised at the ponds until 5 months post-hatching. DNA extract based on standard method and these individuals selected randomly for molecular analysis. Two single nucleotide polymorphisms (SNPs: g.660T>C and g.1904C>A) in intron 1 and 2 regions of the MSTN gene in rainbow trout by were identified DNA sequencing and polymerase chain reaction single-stranded conformational polymorphism (PCR-SSCP) methods. The assessment results demonstrated that 6 haplotypes were revealed in this center. Moreover, association analysis indicated that the genotypes of SNP g.1904C>A are associated with the length and body weight at 90 and 150 days of age (PC and growth traits. Our results provided evidence that SNPs in MSTN gene may be associated with length and body weight at fingerling age and MSTN gene may be used as potential candidate gene for marker-assisted selection (MAS) in rainbow trout breeding program
Keywords:
Language:
Persian
Published:
Iranian Scientific Fisheries Journal, Volume:25 Issue: 3, 2016
Pages:
25 to 39
https://www.magiran.com/p1601484
سامانه نویسندگان
مقالات دیگری از این نویسنده (گان)
-
Effects of short-term adaptation of weaning strategies on growth performance, survival rate, carcass composition, and fatty acids profile of great sturgeon (Huso huso) larvae
Zabihollah Pajand*, Reza Taati, Mahmoud Mohseni, Hosseinali Abdolhay, Reza Ghorbani Vaghei, Zhila Khodkhah
Iranian Scientific Fisheries Journal, -
Study of the growth pattern of kutum (Rutilus kutum Kamensky, 1901) in SefidRood River - Gilan Province
Nasser Karami Rad, Hojatullah Jafarian *, Rahman Patimar, Hossein Ali Abdulhai, Mohammad Farhangi
Journal of Utilization and Cultivation of Aquatics, -
Interspecific gynogenesis induction in Siberian sturgeon (Acipenser baerii ) using heterologous sperm
Mohammad Hassanzadeh Saber *, Shahrouz Baradaran Noveiri, , Mahtab Yarmohammadi, Hooshang Yeganeh Raste Kenari, Jalil Jalilpoor
Physiology and Aquatic Biotechnology, -
Identification of the most important environmental and management factors affecting on Salmo trutta caspius Aquaculture duration
Maryam Nafari Yazdi*, Hosseinali Abdolhay, , Ardeshir Nejati Javaremi
Journal of Aquaculture Development,