Effects of biological treatments on disease intensity reduction of wheat brown rust caused by Puccinia reconditeRob. ex. Desem f.sp. tritici was studied in a split-split plot design, conducted based on completely randomized design in a glasshouse with three replications. Chamran and Boolani cultivars planted in main plots and three Trichoderma species including Trichoderma harzianum, T. citrinoviride and T. longibrachiatum were located in first subplots and leaf and seed treatments inoculation methods were used in second subplots. Changes in peroxidase enzyme level were also determined by Hemeda and Kelin method. The results showed a significant difference between chamran and boolani cultivars (P