Effects of culture media and growth regulators on propagation of rootstock GF677 in tissue culture conditions

This research was investigated the effect of growth regulators and culture media on micropropagation potential of GF677 rootstock. This experiment was conducted during 2012 at the laboratory of plant tissue culture "Razavi Khorasan Agricultural and Natural Resources Research Center" using a RCB design with three replication .In this Research, three culture media containing MS, WPM and DKW supplemented with: 0, 0.5 and 1 mg/l benzylaminopurine (BAP) and with same combination of naphthalene acetic acid (NAA) was examined on proliferation potential of GF677 rootstock. In the end of experiment, the mean of leaves, shoots and roots number as well as stem and root length, was measured. Results of variance analysis showed that there were significant differences among culture media, hormone and their interactions for all traits studied. Best answer for all studied traits was obtained of MS and WPM, while DKW was the weakest response of the environment. Interaction effects showed that the highest value for number of leaf per shoot, shoot length and shoot number were obtained in WPM medium supplemented with 1 mg/l BAP without NAA. In this experiment, the highest mean root number and root length per shootlet were obtained in the WPM medium supplemented with o.5 mg/l NAA.