The effect of different concentrations of hormones and explant type on callus induction on persian poppy

According to the in vitro advantages compared to conventional method, the effect of different concentrations of plant growth regulators (2,4-D and BAP) and explants (root, leaf and crown) on callus production in Iranian poppy plants by measuring the fresh weight, callus volume and callus surface was studied. The present study performed on base of factorial experiment in a completely randomized design with three replications. According to the obtained results, the highest callus fresh weight (1.52 g), the highest callus surface (1.25 cm2) and the highest callus volume (0.562 cm3) related to the root explant in hormone treatment of 0.5 mg/l 2,4- D and 0.5 mg/l BAP. The lowest fresh weight (0.061 g) observed in concentration of 1.0 mg/l 2,4-D and 1.0 mg/l of BAP and the crown explant. The lowest surface of callus (0.13 cm2) for root explant was obtained at 0.5 mg/l 2,4-D and 1 mg/l BAP and the lowest callus volume (0.008 cm3) was owned by root explant in hormone concentration of 0.1 mg/l 2,4-D and 0.1 mg/l BAP.