In silico fusion of epsilon and alpha toxin genes of Clostridium perfringens type D and Clostridium septicum

Recombinant DNA technology is an in vitro molecular techniques to isolate and manipulate DNA fragments. Using this technique, construction chimeric molecules, called recombinant DNA molecules, then insert the recombinant molecules to living cells, replication and proliferation of the cells was possible. The fusion protein technology is the strategy to achieve rapid, cheap and efficient expression of proteins. Clostridium perfringens type D epsilon gene nucleotide sequence (etxD) and Clostridium septicum alpha gene (csa) were retrieved from GenBank. Then, to produce chimeric fusion protein, epsilon-alpha fusion gene was designed. Secondary and tertiary structures and characteristics of the fusion protein was determined by online software. The results showed that the designed fusion gene construction is suitable to expression of chimeric fusion protein.