Molecular detection and antibiotic susceptibility of Ornithobacterium rhinotracheale in slaughtered broiler flocks of Golestan province

Ornitobacterium rhinotracheale (ORT) is a gram-negative bacterium which causes respiratory disease and reduced flock performance in commercial poultry farms. The aim of the present study was molecular detection and determination of antibiotic susceptibility of ORT in broiler flocks of Golestan province. In this study, 513 samples were taken from trachea, lung and infraorbital sinuses of 50 broiler flocks from 9 poultry abattoirs of Golestan province. Out of 29 flocks(58%) were detected against to Ornitobacterium rhinotracheale infection with using specific primers of ORT in PCR method was amplified A 784 bp fragment of the 16SrRNA gene. Antimicrobial susceptibility test using standard disk diffusion technique was performed with 16 antibiotics. All isolates were susceptible (100%) to: Florfenicol, Tiamulin, Tylosin, Penicillin, Amoxicillin, Ampicillin. Also100% of the isolates were resistant to: Erythromycine, Streptomycin, Gentamycin, Colistin, Neomycin, Sulfamethoxazole Trimetoprim, Bacitracin. The results showed that prevalence rate of ORT infection was high in Golestan province.