Detection of bla TEM, bla CTX-M and blaSHV in Escherichia coli isolated from poultry by multiplex-PCR and determination of the strains susceptibility profile in Kerman province

Colibacillosisis one of the common illness in the Broiler chicken farms that increase resistance and overuse of drugs in recent years has brought a lot of losses. The aim of the current study was the detection of bla TEM, bla CTX-M and bla SHV in Escherichia coli isolated from poultry by multiplex-PCR and their antibiotic susceptibility profile. A total of 60 isolates of E. coli were collected from the poultry. The antibiotic susceptibility test was determined using the disk diffusion method agreeing with CLSI guideline. Cellular DNA was extracted by CinnaPure-DNA (Cell culture, Tissues, Gram negative Bacteria and CSF) and MPCR was performed for the identification of the CTX-M, SHV and TEM genes.
The antibiotic susceptibility test results showed that, the lowest and highest resistance rate were related to imipenem (0%) and aztronam (77%) , respectively. 41(68.3%) , 28(46.6%) and 0(0.0%) strains were positive for CTX-M, TEM and SHV, respectively. 16(26%) isolates carry out both TEM and CTX-M. Disk diffusion method for laboratory diagnosis of ESBLs is highly functional and cost-effective method that can be given to the importance of ESBLs-producing organisms to test routine is added. In addition, PCR assay for genotyping can be used.