Essential oil composition of Achillea millefolium subsp. millefolium in hydrodistallation and Microwave apparatus

In this study aerial parts of Achillea millefolium subsp. millefolium in bloomingwere, collected on summer 1390 from Research station of Research Institute of Forests and Rangelands in Alborz Province. Essential oils were extracted by, hydro-distillation and microwave apparatus methods. Oils yields were 0.4% in hydro-distillation and 0.2% in microwave methods, which reason for low efficiency of essential oil was condition of time. Essential oils composition were analyzed by gas chromatography (GC) and gas chromatography connected to a mass spectrometer (GC/MS). In hydro-distillation, the major compositions were γ-terpinene 18.2%, elemol acetate 12.9%, caryophyllene acetate 12.2% and bornyl acetate 10%, respectively, but in Microwave method 1-terpineol 17.6%, α- bisabolol oxide A 10.6%, trans-thujone 7.3% and terpin-4-ol 7.1% were the major constituents, respectively. The results of the two methods has shown a lot of changes in quantitative and qualitative by Microwave apparatus.