The effect of vitamin B12 on bases of Tris extender on protection of Zel ram spermatozoa

Vitamin B12 is water-soluble vitamins that are essential in the process spermatogenesis and survival of normal function. We have little information about supplementation of vitamin B12, on quality of frozen semen. So the aim of this study was to investigate the effects of different levels of vitamin B12 on semen quality of Zel ram spermatozoa in pre and post freezing conditions.
Samples were collected from 6 healthy and mature rams with 55± 5 Kg body weight and 2-3 years old by using electro-ejaculator. Suitable samples were mixed with Tris extender in a ratio of 1:4 semen and after cooled to 5-00C was assessed. Then, 0.25 ml of straws was filled with diluted semen. At first, straws put upper vapor liquid nitrogen for 6-8 min, then kept and frozen in -196oC. After 10 days straws were thawed in 37oC and viability, motility, progressive motility, morphological defects, acrosomal defects, normal spermatozoa, plasma membrane integrity and recovery rate of viability and motility of spermatozoa by using staining and microscopic evaluation was performed and the antioxidant enzymes catalase, glutathione peroxidase and superoxide dismutase of semen were measured. This experiment was carried out on the basis of completely randomized design with 4 treatments including levels of vitamin B12 (0, 1, 2 and 3 mg/mL) and 8 replications. The data from these tests was analyzed by using SPSS software, version 15.
Results showed that effect of vitamin B12 on vital parameters of spermatozoa such as viability, progressive motility, membrane integrity, defect acrosome and normal spermatozoa at cooled to 5oC and post frozen conditions were significant (p Therefore, it could be suggested that the level of 2 mg /ml, vitamin B12 in Tris extender can be used to protect spermatozoa and prevent structural damage caused by free radicals and lipid peroxidation and improve antioxidant enzyme activity in the process of frozen thawed of Zel ram semen.