Comparison of various methods of Corrosion Cast Study in Silver Carp (Hypophthalmichthys molitrix) Circulatory System

Corrosion cast study is one of the procedures for studying the structure of the blood vessels in a particular tissue or organ. In the present study, the circulatory system of Silver Carp was studied by using four different methods of resin injection. 12 fish with a weight range of 850 -1000 g were obtained from a commercial fish farm. The Fish were anesthetized using a solution of 5% benzocain in 96% ethanol and injected intraperitoneally with heparin (4000 IU/kg). After 40 min, the fish were killed using an overdose of the benzocain solution, and then, fluid artificial resin made on the basis of methylmetacrylate was injected through the caudal artery, bulbus arteriosus, and dorsal and ventral aortas. The Fish were submerged for 24-48 hours in a bath water at room temperature until the polymerization and hardening of methylmetacrylate. Then, they were placed in a 25% KOH solution for 24-48 hours to obtain a maceration of the organic tissue. In this study, various parts of the heart and its vessels, gill vessels, gastric vein, portal vein, hepatic vein, dorsal aorta and its branches, renal and caudal arteries and related smaller vessels were also determined. Overall, it seems that the injection of resin via bulbus arteriosus of the heart was the best and most effective method for the corrosion cast study of the circulatory system in Silver Carp.