Optimization of enrichment method for Artemia urmiana nauplii with probiotic bacteria (Bacillus subtilis and Lactobacillus rhamnosus)

Artemia is a good vector for transferring nutrient substances and different compound such as drugs¡ etc¡ into the aquatic larvae. The aim of this study was to optimize the enrichment method of Artemia urmiana nauplii with two probiotic bacteria¡ Lactobacillus rhamnosus and Bacillus subtilis. For the same purpose¡ at first stage¡ the effects of different salinities¡ temperature¡ and number of bacteria on enrichment of Artemia were studied. In the next stage¡ the best concentration of probiotics for enrichment of Artemia nauplii on the basis of first stage observation was investigated. The result obtained from this study revealed that optimum conditions¡ such as salinity¡ temperature¡ time intervals for addition of bacteria and number of probiotics are 30 ppt¡ 32˚C¡ 6 h and 106 CFU/ml for Lactobacillus rhamnosus and 40 ppt¡ 28˚C¡ 9 h and 107 CFU/ml for Bacillus subtilis¡ respectively. The result obtained from this study revealed that optimum conditions¡ depend on bacterial type was alter. On the basis of these findings¡ we conclude that in order to obtain maximum enrichment of a particular probiotic specific enrichment conditions must be provided.