Introducing standard protocol for enrichment of Artemia franciscana nauplii with Canola oil

In this study¡ the effects of enriching Artemia franciscana nauplii with three concentrations of Canola oil at three density levels were evaluated on survival¡ total length and fatty acids of Artemia franciscana nauplii to determine the optimum concentration oil¡ density of nauplii and optimum time for maximum survival rate and fatty acids and minimum total length of nauplii. Cysts of Artemia franciscana were hatched according to the standard method (Sorgeloos¡ 1986). Artemia franciscana nauplii were then stocked at densities of 50000¡ 100000 and 200000 nauplii L-1 in 7 L cylindrical containers. Canola oil emulsion was added at concentrations of 0.1¡ 0.2 and 0.3 g L-1 at the beginning and 12 hours after the onset of enrichment. Nauplii were sampled at 6¡ 12 and 18 h after onset of enrichment for measuring the survival¡ total length and the concentration of fatty acid. The results of analysis showed that enrichment of Artemia with 0.3 g L-1 Canola oil at 50000 nauplii L-1 for 18 hours was considered as best treatment. Artemia nauplii enriched in this treatment had maximum linoleic and linoleic acids¡ highest survival and minimum total length.