Effect of combination of high-dose phytase, citric acid and carbohydrases on performance of broiler chickens fed wheat-canola meal-based diets with very low content of non-phytate phosphorus

An experiment was conducted, from 22 to 42-d-post-hatch, to evaluate the effect of different combinations of carbohydrases (CHS), citric acid (CA) and high-dose phytase in wheat-canola meal-based diets with very low content of non-phytate phosphorus (NPP) on growth performance, plasma calcium (Ca) and P and tibia ash (TA) content of broiler chickens. One hundred and ninety two 21-d-old male chicks were allocated into 24 pens and fed one of six dietary treatments. The dietary treatments were T1) a negative control (NC, 1.68 g/kg NPP), T2) NC CHS (500 mg/kg) 2,000 phytase FTU/kg, T3) NC CHS CA (20 mg/kg) 2,000 phytase FTU/kg, T4) NC CHS 4,000 phytase FTU/kg, T5) NC CHS CA 4,000 phytase FTU/kg or T6) a positive control (PC, 4.2 g/kg NPP). The birds fed on PC had higher average daily gain (ADG, P