effect of methanolic extracts medicinal plants Quercus brantii, Pistacia atlantica and Elaeagnus angustifolia on biofilm formation Pseudomonas aeruginosa

Biofilm formation is one of the most notable mechanisms that helped antibiotic resistance in Pseudomonas aeruginosa , The aim of this study were effects of methanol extracts of Quercus brantii jaft, Pistacia atlantica, Elaeagnus angustifolia Leaves on biofilm formation Pseudomonas aeruginosa strains.
this study is an experimental study, that extraction with Maceration(powder mixed with 80% methanol ) and rotary evaporator were done. The minimum inhibitory concentration extracts were done by broth microdilution. biofilm formation by microtiter plate method and stained with crystal violet was used. Data collection were analyzed by SPSS software.
The minimum inhibitory concentration Quercus brantii jaft, Pistacia atlantica, Elaeagnus angustifolia extracts against Pseudomonas aeruginosa showed .625 mg/ml , the Minimum bactericidal concentration of Quercus brantii jaft 1.25 mg ml and for Pistacia atlantica, Elaeagnus angustifolia showed 2.5 mg ml. average percent of Pseudomonas aeruginosa biofilm inhibition by extracts of Quercus brantii jaft, Pistacia atlantica, Elaeagnus angustifolia were 60.24, 57.35, 72.63 % Respectively
Methanolic extracts of medicinal plants Quercus brantii, Pistacia atlantica, Elaeagnus angustifolia It has anti-bacterial and anti-biofilm effect Were against Pseudomonas aeruginosa So that Elaeagnus angustifolia extract Better ability to inhibit biofilm was and to extracts of Quercus brantii jaft, Pistacia atlantica significant differences Showed and in the future we can used thes extract with more work as complementary with antibiotics.