Effects of growth regulators on in vitro callogenesis and regeneration of black cumin (Nigella sativa)

Black cumin (Nigella sativa) is one of the most important medicinal plants belongs to Ranunculaceae. In spite of its significance in pharmaceutical industry, tissue culture and micropropagation of the species is not well studied yet. Hence, based on the issue we aimed to study its callogenesis and regeneration on leave explants of the species. In vitro provided explants of leave were cultured on MS medium supplemented with NAA (0.5, 1, 1.5 mgL-1) and BAP (0.2, 0.5, 1 mgL-1). Half MS medium supplemented with 0.5, 1 and 1.5 mgL-1NAA and IBA were used for rooting the regenerated shoots. Results showed that higher percentage of callogenesis was observed on MS medium supplemented with 0.5 mgL-1NAA and 0.5 mgL-1 BAP. Shooting was observed on MS medium (1mgL-1BAP) and rooting on half MS (1 mgL-1NAA), respectively. Results indicated that callogenesis, shooting and rooting of black cumin are highly affected by hormonal combination levels.