Study of Genetic Variation in Iranin rice (Oryza sativa L.) using SSR Markers

In order to study genetic diversity in rice genotypes In Iran, the variation in 38 rice cultivars were evaluated using 14 SSR markers. The experimental design was a randomized complete block at the research station located in the city in 1392 was Azadshahr. Plant samples at tillering genotypes were collected in the early morning. DNA was extracted from plant samples denaturation was carried out on polyacrylamide gels. Large genetic distances between cultivars showed high genetic diversity among the genotypes of rice. Principal component analysis of variance was 62%. The highest polymorphic information content RM262 (81/0) and lowest at RM241 (33/0) were recorded and the average PIC, 51/0 respectively. 33/0 Shannon's index of microsatellite markers used in this study, markers RM142 RM255, RM341, RM262, had high discriminatory power. The highest number of positive markers in height (11), respectively. Genotypes and cluster analysis methods into three classes, respectively. In general we can say the numbers in the high country of diversity and diversity in them can be used in breeding programs. Markers used in this study due to the high variability in their suffering, would be useful in future studies or remedial work.