Effects of different centrifugation protocols on pre and post-cooling sperm quality in Caspian horses

This study aimed to determine the effects of different centrifuging protocols (different rotation and time) on Caspian horse sperm characteristics. In this study, semen samples from four healthy adult Caspian pony stalian were collected using an artificial vagina and then diluted in milk phosphocaseinat based extender. Then semen samples were divided into seven equal parts, which part assigned to treatments including centrifugation treatments (500×g, 1000×g and 2000×g each for 4 and 7 minutes) and without centrifuge (control). Cooling of treatments were done at 0, 24 and 48 hours after centrifugation. Sperm motion characteristics, viability and percentages of healthy sperm were assessed. The results showed that motility, viability and percentages of healthy sperm reduced in 2000×g treatments (p