Isolation of Alicyclobacillus from fruits juice in Iran

Heat-loving bacteria Bacillus Alicyclobacilluschanging flavor juices and ready to use in recent years in a wide range of large amounts of fruit juice was corrupt world, although there is no previous report indicating its pathogenesis. This research was done to study contamination of packaged juice with the bacteria. A total of 300 samples of pomegranate juice, apple juice, orange juice, mangoes juice, pineapple juice, grape juice (from different companies) at Esfahan was collected and studied for Alicyclobacillus contamination. The samples were tested using culture, biochemical tests and PCR. According to the results, 13 samples (4.33%) were contaminated with Alicyclobacillus. The highest level of contamination among samples was found in pomegranate juice and orange juice (13.3%). Contamination in cranberry juice, mixed species and other samples was 10, and 6.66 %. No contamination was found the other species. Regarding to the obtained results, prevention of bacterial growth especially during storage time and detection of bacteria after pasteurization is necessary.