Improvement of in vitro micro propagation of Rosa damascena genotypes

Rosa damascena is one of the most important Aromatic plants in the world. The extracted oil from the petals of the flowers is used in the pharmaceutical, cosmetic and food industries. Traditional methods to propagate this plant have problems such as transfer of pollution agents and limited native plants. Due to more advantages of the in vitro proliferation in compared to traditional methods, this study designed to evaluate impression different medium cultures with diverse hormone levels to improve the proliferation and control of shoots chlorosis. In this research we used medium cultures of MS and mMS (mMS0, mMS1, mMS2, mMS3, mMS4 and mMS5). In order to establishment in MS medium, nodal segments containing 1 or 2 lateral buds were cultured. For regenerating the shoots, mediums with different concentrations of 1 and 2 mg/l BAP and 0/2 mg/l IBA were supplied, then plantlets were transferred to the rooting medium (1/2 MS and LS) with different levels of IBA (1 and 2 mg/l). The best treatment to increase the number of rose shoots was mMS medium containing 1.5 mg/l of BAP and 0.2 mg/l of IBA. The highest chlorophyll content of leaves was observed in mMS medium. Gallica plantlets produced the highest percentage of rooting on 1/2 MS with 2 mg/l of IBA.