Comparison of Direct and Indirect Regeneration Methods in Producing Leaf Buds in Sugar Beet

Sugar beet is an industrial important crop plant in Iran and the rest of the world. Leaf buds emerging on leaves are the most important explants used in the transformation of sugar beet. In order to study the production of leaf buds by direct and indirect organogenesis in sugar beet, an experiment was carried out as factorial in a randomized complete design with four replications. In direct regeneration method, Benzyl adenine (BA, 0.5 mgL-1) and Naphthalene Acetic Acid (NAA, 0.5 mgL-1), and in indirect method 2-4-D (0.5 mgL-1) and kinetin (Kin, 0.5 mgL-1) along with two sugar beet lines (SBSI-04 and SBSI-02), three types of leaf explants, including terminal bud, leaf and petiole, were used. Analysis of variance (Anova) showed that SBSI-02 line produced the highest rate of regeneration. Furthermore, the effect of explant type, line and regeneration method on plantlet regeneration was significant (P ≤ 0.01). Regeneration efficiency was also influenced by the line× explants interaction (P ≤ 0.01). The results of this experiment showed that although the regeneration method had a significant effect on the efficiency of regeneration, but there was no significant difference between direct and indirect regeneration methods.