Genetic variability of Teucrium polium ecotypes using ISSR molecular markers

Golden germander (Teucrium polium L.) is an important medicinal plant of the Labiatae family. Genetic variability was studied on 17 ecotypes of Teucrium polium collected from Kermanshah, Iran, based on ISSR molecular markers. Twelve ISSR primers out of 15 primers could be scored. ISSR primers produced 82 bands, of which polymorphism was observed on 80 bands. Average number of bands was 6.83 for each primer. Primer IS6 showed the highest number of bands (12 bands) and IS3 showed the lowest number of bands (4 bands). The results indicated that ecotype G1 had the most genetic distance with G12, G7, G13 and G8. Based on cluster analysis the ecotypes were grouped into four clusters. Genetic diversity of ecotypes did not match with the geographical diversity of the ecotypes. Also results of grouping by analysis of molecular variance (AMOVA) and coordinate analysis (PCo) were confirmed.