Prevalence of Beta-lactamases Genes and Antibiotic Resistance Pattern of Klebsiella pneumoniae Isolated from Teaching Hospitals, Sari, Iran, 2014

Background and : Klebsiella pneumoniae has emerged as a significant opportunistic pathogen responsible for nosocomial infections. This bacteria is frequently resistant to multiple classes of antibiotics such as family of beta-lactam antibiotics. β-lactamases enzyme represent the main mechanism of bacterial resistance to β-lactam antibiotics. This study was conducted to determine the prevalence of TEM-1and CTX β-lactamases in Klebsiella pneumoniae isolates from the hospitals in sari, Iran.
The study included 45 Klebsiella pneumoniae isolates that were isolated from various clinical specimens. Susceptibility of isolates to antibiotics was determined by standard disk diffusion method. ESBL production was determined by combination disk method. The resistance of TEM and CTX types of ESBL producing genes was identified by PCR test using specific primers. Data analysis was done in SPSS V16 applying Chi-square test.
Among all Klebsiella pneumoniae isolates, the highest resistance was seen against cefotaxime (100%) and ceftazidim (100%), while the highest susceptibility was observed to gentamycin (63%). According to the results, 60% of isolates were ESBL positive and among them 55% and 45% were positive for blaTEM and blaCTX genes, respectively.
: In this study drug resistance of Klebsiella pneumoniae isolates was observed in 60% of blaTEM and blaCTX genes, therefore, other mechanisms such as efflux pumps and biofilm formation can have a role in drug resistance.