Anti-inflammatory effects of novel thiazolidinedione derivative on RAW 264.7 cells stimulated with LPS

Previous data have shown that the thiazolidinediones and their derivatives are found to possess divergent and very interesting biological activities such as, antioxidant and antibacterial activities. In this study, we investigated the cytotoxicity and inhibitory effects of synthesized TZD-OCH2CH3 on lipopolysaccharide (LPS)-induced over-expression of inducible nitric oxide synthase (iNOS) using Raw 264.7 cells. To assess whether the tested TZD-OCH2CH3 affected cell viability, RAW 264.7 cells were incubated with LPS (1μg/ml) in the presence of different TZD-OCH2CH3 concentrations (0-300 μg/ml). The results of MTT assay showed that TZD-OCH2CH3 had cytotoxicity at higher concentrations of 50 μg/ml (IC50=120 μg/ml). We then further evaluated the inhibitory activity of different TZD OCH2CH3 concentrations (30 and 60 μg/ml) against NO production in LPS-stimulated RAW 264.7 cells. The synthesized TZD-OCH2CH3 was found to have the most significant inhibitory effect on NO production in LPS-induced RAW 264.7 cells. These findings demonstrated that TZD-OCH2CH3 has good anti-inflammatory activity and thus has great potential for use in the control of various inflammatory diseases.