Single nucleotide polymorphisms of MMP2 gene promoter on the risk of lung cancer development and metastasis

The high incidence and poor prognosis of the lung cancer makes it a major health problem in the last few decades. Determination of frequency of different histopathology types of primary lung cancer has great importance in creating integrated treatment programs and to recognize the effective factors causing the disease. Overexpression of MMPs has a direct relation with invasion and metastasis of malignant tumors in different tissues. The aim of this study was to effect polymorphism in MMP-2 gene with suffering to lung cancer and its metastasis in case and control groups by PCR-RFLP.
In the present case-control study, The MMP-2 polymorphism was analyzed by restriction fragment-length polymorphism (RFLP) in 50 patients with lung cancer and 77 cohort sample. All samples were examined by a specialist doctor. DNA isolation was performed using DNA extraction kit (Cinnagen, Iran). MMP9 gene was amplified by specific primers and PCR product was digested with FSPBI restriction enzyme. Data analysis was used by Chi square SPSS software.
The examination of allelic distribution and genotypic distribution in patients with lung cancer and control showed that the allele frequency of C and T in patients with lung cancer were 90 and 10 (P=0.04) and in the control were 80/15 and 30 (P=0.05) percentage respectively. Also genotype frequency of CC, CT and TT in patients with lung cancer were 82, 16 and 2 (P=0.05) and in the control were 69/93, 31/16, 3/1 percentage respectively (P=0.5). Comparison of non-metastatic genotypes and control groups did not showed a statistically significant difference (P=0.5), but compared to CC genotype in metastatic and control groups (P=0.05) allele C directly involved in the metastasis.
It seems that individuals with C allele can increase susceptibility to lung cancer. Also these findings indicate that CC genotype as a risk factor facilitating the spread of metastasis of lung cancer.