The effect of In ovo Injection of Calcium, Phosphorus, and Vitamin-D on Hatchability, Blood biochemical Parameters and Bone of Broiler Chickens

The effect of In Ovo Injection (IOI) of calcium (Ca), phosphorus (P), and vitamin D (VitD) was studied on hatchability, post-hatch bone characteristics, and broiler chicks performance in two experiments. In first experiment, the 1080 fertilized eggs (Ross strain) were randomly distributed into 9 groups. Treatments were 1- positive control (injection of physiologic serum) 2- negative control (without injection of solution), 3- injection of Ca solution, 4- injection of P solution, 5- injection of VitD solution, 6- injection of Ca×P, 7- injection of Ca×VitD, 8- injection of P×VitD solution, 9- injection of Ca×P×VitD. After hatched, 80 chicks of each experimental group were selected and in reared under completely randomized designed (4 replicate of with 20 birds) for 6 weeks. The hatchability percentage increased by IOI of Ca×P×VitD (P