Neurogenic Differentiation of Rat Bone Marrow Stromal Cells by the Non Toxic Factors of Bioactive Substance as an Inducer
Author(s):
Abstract:
Introduction
Despite major progress in pharmacological and surgical approaches, spinal cord injury remains a complex medical challenge. Cell replacement therapy is one of the new approaches for spinal cord injury treatment. In this study, bioactive substance TNT (a new supplement) was utilized for induction of bone marrow stromal cells (BMSCs) into neural stem cells (NSCs).Materials And Methods
The BMSCs were extracted and cultured from the femurs and tibias of adult female wistar rats. After 3 or 4 passages, these cells were preinduced into neurospheres by TNT and neural induction supplement. Then neurospheres were induced into NSCs with B27 and TNT. The NSCs were evaluated with nestin, NF68 and SOX2.Results
The outcomes indicated that BMSCs were immunoreactive to CD106 (95.7 ± 0.76), OCT4 (96.2 ± 1.3) and CD45 (0.4 ± 1.2). The optimal dose and time for application of TNT were 1 M and 6 days, respectively. Differentiated cells were able to express nestin and NF68.Conclusion
TNT as a non-toxic substance may be a good alternative for cell induction into NSCs for treatment of neurodegenerative disorders.Keywords:
Language:
Persian
Published:
The Neuroscience Journal of Shefaye Khatam, Volume:2 Issue: 2, 2014
Pages:
47 to 55
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