The Effect of Growth Regulators on Callus Induction and Regeration in citrus Rootstock

One of the main objectives of citrus rootstock breeding is plants free of disease. In order to evaluate the effect of growth regulators on callus induction and regeneration of citrus rootstock, five citrus rootstocks were selected. Seeds were germinated after one month. Culture were incubated at 25 ° C and exposed to 16 hours per day. Explants were cultured on EME medium. Callus induction under factorial experiment with five citrus rootstock (Sour orange , Citrange, Citromelo, Poncirus, Volkameriana), concentration of NAA in three levels (0.5, 1, 1.5 mg) and concentration of Kin in three levels (3, 4, 6 mg) in a completely randomized design with three replications. The callus regeneration, five citrus rootstock with five hormonal treatment of factorial experiment in a completely randomized design with three replications was used. The results showed that the highest percentage of callus induction Sour orange of 0.5 mg/l NAA three mg/l Kin (25 %), the highest percentage of callus induction poncirus of one mg/l NAA three mg/l Kin (100%) and 1.5 mg/l NAA three mg/l Kin (100 %). highest percentage of callus induction for Citrange on the 0.5 mg/l NAA six mg/l Kin (91%) and Citromelo of one mg/l NAA three mg/l Kin (92%) and the highest percentage Volkameriana callus induction to 1.5 mg/l NAA three mg/l Kin (100 %). Frequency of callus induction with NAA and Kin (growth regulators)at concentrations of 1.5 and three mg/l. BA and NAA are known to be effective in regeneration. three mg/l Kin and 0.2 mg/l BA one mg/l NAA increased callus induction and regeneration, respectively.