The effects of blue and red light on the expression of CRY1 and HY5 genes in rape (Brassica napus L.) seedlings

In recent decades, several researches have been done on plants in order to evaluate the expression of genes influenced by blue and red light. The aim of present study is analyzing the effects of blue and red light on the expression of CRY1 and HY5 genes in rape seedlings. These seedlings were placed in steady environmental conditions and then in blue light treatment (2, 4 or 8 hr) and in red light treatment (2, 4 or 8 hr) for 5 days, and the variation of hypocotyl length in various treatments was scrutinized and the expression level of CRY1 gene was analyzed by Semi-quantitative Reverse Transcription-Polymerase Chain Reaction (RT-PCR) technique. Also, the amount of HY5 gene expression along with CRY1 gene expression was examined by Quantitative Real-time-Polymerase Chain Reaction (qRT-PCR) technique. Results of studying the effects of blue light treatment on control and treated plants showed that the blue light is capable to adjust expressing CRY1 gene and thus to control photomorphogenesis in rape (Brassica napus L.) plants, so that in an 8-hour treatment with blue light, we observed the highest level of CRY1 expression. Of course, the expression level of HY5 gene reached to its highest point, which resulted in maximum inhibition of hypocotyl elongation in this special group.