Antibacterial Effects of Alyssum homolocarpum L. Aqueous and organic Extracts against some Gram-positive and Gram-negative Bacteria

Alyssum homolocarpum L. common in traditional medicine of Iran. In this research, Antibacterial effects of Alyssum plants were evaluated against a number of bacteria gram-positive and gram-negative.
Extraction plant in the vegetative stage, pre-flowering and flowering with solvents water, ethanol, ethyl acetate, acetone, methanol and hexane by maceration method was performed.Concentration of 50 mg/ml extracts were prepared and evaluation of the antibacterial effects against strains of Staphylococcus aureus, stereptococcus Pneumonia , gram-positive bacteria and E.coli gram-negative bacteria was performed. Antimicrobial effects of aqueous and organic extracts of Alyssumat various developmental stages, first, The diffusion method was tested in Muller -Hinton agar medium and Then minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was determined.
The results showed that the extract prepared from pre-flowering stage¡More inhibitory effect than other aqueous and organic extracts growth of microorganisms is shown. The highest and lowest antimicrobial effect earlier stages of flowering and vegetative respectively. Aqueous extracts of the three stages of plant growth Alyssum had antimicrobial effects on gram-positive bacteria¡ but had little effect on gram-negative bacterium E. coli. Also, little MIC and MBC to extract pre-flowering stage, against gram-positive bacterium Streptococcus pneumoniae (Respectively, 0/75 and 1/5 mg/ml) and most MIC and MBC for vegetative stage of acetonia extracts against gram-negative bacteria E. coli was obtained (Respectively, 55 and 110 mg/ml).