The Effect of Spray Dried Sourdough Process on Gluten Development by Studying Dough Microstructure

Sourdough is a complex biological system, consisting of such components as cereal carbohydrates and proteins, lactic acid bacteria and yeasts and different enzymes from which each of these taste, flavor and shelf life of bread can be improved with optimal use of sourdough. The aim of this study was to determine the effect of spray dried sourdough addition on gluten development through studying dough microstructure using epi-flurescence microscope. Therefore, the traditional dough was prepared by adding 15% sourdough powders containing three lactobacillus strains, which were previously isolated from Iranian traditional sourdoughs (Lactobacillus plantarum, Lactobacillus curvatus and Lactobacillus Paralimentarius), to the dough. According to microscopic observation with simultaneous staining of dough samples with rhodamine 1% and FITC 0.1%, it was showed that samples had continuous gluten network layer and protein network was not destroyed. Which can be attributed to the insufficient amount of microorganisms, low resting after preparing the dough and insufficient activity of microorganisms.