Synthesis of Silver Nanoparticles Using Green Method of Plant Extract European Marjoram (Origanum majorana) and Their Antibacterial Effects

There are many different methods for the production of silver nanoparticles (Ag-NPs) but, using of plants in the synthesis of nanoparticles due to a cost effective and eco-friendly approach has been extensively noticed. In this study, the biosynthesis of silver nanoparticles by using extract of Origanum majorana and its antimicrobial properties has been reported. In this study, the extract of Origanum majorana as the reducing agent is used for the biosynthesis of silver nanoparticles. Reduction of Ag ion was obtained after 24 h by using extract of Origanum majorana in presence of 1 mM silver nitrate (AgNo3) solution. The size, structural, optical and morphological properties of nanoparticles have been analyzed by facility of: X-ray Diffraction, Field Emission Scanning (Transmission) Electron Microscope. The antimicrobial activities of the silver nanoparticles against Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus was investigated. UV-Vis spectrum exhibit an absorption band at around 400-450 nm suggestion the formation of biological Ag nanoparticles. Size and morphological properties of nanoparticles was performed by TEM which show that particles have spherical shape with diameter of about 30-70 nm. Silver nanoparticles presented the antimicrobial activity against Gram positive and Gram negative bacteria. It seems that green Synthesis of nanoparticles using plant extracts can help to increase their antibacterial. In the present study it was shown that the antibacterial properties of nanoparticles to increase considerably.