In Vitro Evaluation of Equine Fibroblast-Like Synoviocytes Viability Treated with Doxycycline
Author(s):
Abstract:
Objective- The purpose of present study was to investigate the viability of equine fibroblast-like synoviocytes (FLSs) treated with doxycycline.
Design- Experimental study.
Sample population- FLSs from metacarpophalangeal joints of six skeletally mature horses.
Methods- FLSs were established from synovial fluids of healthy joints. The cells were treated with different concentrations (1, 5, 10, 50, 100, 150, 300, 400 µg/ml) or without doxycycline for 48-hour. Viability of FLSs was determined by MTT assay and the Trypan blue dye exclusion method.
Results- No significant differences were observed between viability of FLSs cultures treated with doxycycline until 150 µg/ml and control group (P>0.05). Doxycycline at 300 and 400 µg/ml significantly decreased FLSs viability (PConclusion and Clinical Relevance- These findings demonstrate that doxycycline was not toxic for equine FLSs at concentration equal or less than 150 µg/ml in vitro. Further studies are needed to investigate the safety, efficacy and detrimental effects of doxycycline in equine joints.
Design- Experimental study.
Sample population- FLSs from metacarpophalangeal joints of six skeletally mature horses.
Methods- FLSs were established from synovial fluids of healthy joints. The cells were treated with different concentrations (1, 5, 10, 50, 100, 150, 300, 400 µg/ml) or without doxycycline for 48-hour. Viability of FLSs was determined by MTT assay and the Trypan blue dye exclusion method.
Results- No significant differences were observed between viability of FLSs cultures treated with doxycycline until 150 µg/ml and control group (P>0.05). Doxycycline at 300 and 400 µg/ml significantly decreased FLSs viability (PConclusion and Clinical Relevance- These findings demonstrate that doxycycline was not toxic for equine FLSs at concentration equal or less than 150 µg/ml in vitro. Further studies are needed to investigate the safety, efficacy and detrimental effects of doxycycline in equine joints.
Keywords:
Language:
English
Published:
Iranian Journal of Veterinary Surgery, Volume:12 Issue: 1, Winter-Spring 2017
Pages:
11 to 17
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