Optimization of milk proteins- soluble fraction of Iranian native gums conjugate formation via the Maillard reaction
Author(s):
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Introduction
Maillard reaction is a well-known technique for covalent coupling of proteinpolysaccharide which is usually used to improve the functional properties of proteins. Conjugation by the dry heating method is occurred during Amadori rearrangement step in the Maillard reaction where amine groups of the proteins are linked to the reducing end of the polysaccharides. This glycation process leads to the improvement of solubility, foaming and emulsifying properties of proteins (Liu et al., 2012). Protein-based emulsions are sensitive to pH and ionic strength alterations as well as heating and freezingthawing processes. While, joining proteins to high molecular weight polysaccharides protects them against environmental stresses. A wide range of carbohydrates were already utilized to enhance the emulsifying properties of proteinspolysaccharides namely whey protein isolatedextran (Akhtar et al., 2003), β-lactoglobulindextran (Wooster et al., 2006), sodium caseinatemaltodextrin (ORegan et al., 2009), deamidated wheat proteinmaltodextrin or glucose (Wong et al., 2011), whey protein isolatepectin (Xu et al., 2012), yolk phosvitindextran (Chen et al., 2014), β-lactoglobulinsix-carbon monosaccharides (Cheetangdee et al., 2014), soy protein isolatesoy soluble polysaccharides (Yang et al., 2015), lysozymetragacanth (Koshani et al., 2015), and β-lactoglobulinPersian gum (Golkar et al., 2015). However, based on the existing literature, it seems that conjugation of Iranian native gums and proteins needs to be more attended to show their potential applications. Therefore, in this study, conjugate formation between milk proteins (sodium caseinate and whey protein isolate) and soluble fraction of Iranian native gums (gum tragacanth and Persian gum) was optimized using response surface methodology (RSM) and the resulting conjugates were used in emulsion formulation in order to compare protein capability before and after being attached to the gums.Materials And Methods
Iranian native gums were pulverized and sieved (mesh size Results and Discussion
Based on our findings, the decrease of free amino group indicated that NH2 group of amino acids in milk proteins was covalently linked to carbonyl group of the gums. Comparing free amino group reduction of the samples containing soluble fraction of gum tragacanth and Persian gum also showed that Persian gum was more capable of forming covalent linkage with milk proteins than gum tragacanth; probably, due to the lower side branches of Persian, its molecular weight as well as its structural flexibility. Moreover, we believe the higher side branches of attached-tragacanthin molecules might prevent further attachment of protein to the other polysaccharide molecules via steric repulsion. According to the results of color measurement before and after dry Maillard reaction, conjugation led to reduction of L* and increase of a* and b*. In addition, higher heating time and protein:polysaccharide ratio caused progress of Maillard reaction (especially in the case of milk proteinssoluble fraction of Persian gum). Based on our findings, in dry Maillard reaction, the optimum heating time and protein:polysaccharide ratio were 8 days and 1:1.59 for whey protein isolatesoluble fraction of Persian gum, 7 h and 1:2 for sodium caseinatesoluble fraction of Persian gum, 13.64 days and 1:3 for whey protein isolatesoluble fraction of gum tragacanth, and 7.82 h and 1:3 for sodium caseinatesoluble fraction of gum tragacanth, respectively. Besides, the obtained Maillard reaction products did not cause complete stability of emulsions at pHs 3, 5 and 7. Our findings also declared that attachment of polysaccharides to proteins might negatively affect the proteins functionality, as emulsifier, possibly by hindering adsorption of hydrophobic groups of proteins to oil droplets. Furthermore, homogenization process during emulsion preparation using ultrasound could break the formed covalent bonds and polysaccharide structure leading to lower steric repulsion and viscosity.Keywords:
Language:
Persian
Published:
Iranian Food Science and Technology Research Journal, Volume:13 Issue: 5, 2017
Pages:
744 to 758
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