Investigating Antibiotic Resistance and The Frequency of SHVand TEM Extended Expecterum Beta Lactamase Genes in klebsiella penumoniea Isolated from Blood Samples of Neonates Admitted to Some Health Centers in Rasht

Klebsiella pneumoniae is one of the most common causes of nosocomial infections. The aim of this study was to determine the frequency of the blaTEM and blaSHV genes in K. pneumoniae strains isolated from blood samples of neonates admitted to some health centers in Rasht.
In this study, 163 isolates from blood samples were taken during 6 months from July to December 2015. Antibiotic susceptibility was conducted by disc diffusion method and screening test using combination disc method. Also, the presence of blaTEM and blaSHV genes were studied using PCR method.
Results and Among the total of 163 cultured blood samples, 16 were positive. Only 3 strains were diagnosed as K. pneumonia (18.75%). Two out of 3 positive samples (67%) were identified as the ESBLproducers in the primary screening test. The isolated K. pneumonia had the highest resistance to penicillin and amoxicillin (100%) and the lowest resistance to imipenem and ceftazidime (33%). The results of PCR on 3 samples showed that all the 3 isolates (100%) contained SHV gene, 1 strain (33.3%) had TEM gene and 1 case (33.3%) had both TEM and SHV genes simultaneously. In conclusion the horizontal gene transfer among bacteria in hospitals and clinical centers and the patterns of antibiotic useage should receive more attention.