Quorum-Sensing Signaling System in Pseudomonas aeruginosa: A Method to Overcome the Bacteria's antibioticResistance By Identifying Dual Inhibitors and Using NSAIDs in Combination with Antibiotic Against Biofilm

Nonsteroidal anti-inflammatory drugs (NSAIDs), are used as analgesic and antipyretic agents. In this study NSAIDs were used to overcome the resistance of Pseudomonas aeruginosa biofilms to Tobramycin antibiotics. The aim of this study was to find an easy and low-cost method, using NSAIDs and Tobramycin, to deal with the biofilms of P. aeruginosa..
Follwoing molecular remodeling, NSAIDs were used alongside Tobramycin against PAO1 strain of P. aeroginusa . The Plate Reader measured the absorbance of crystal violet solution in ethanol. The Infiltration rate of NSAIDs in biofilms and its absorbance were measured by Tetrazolium salt and Plate Reader, respectively.
After performing the docking techniques, the most suitable conformation of NSAIDs, to inhibit LasR and PqsE proteins, were chosen. Having worked on dual inhibitors, we could find a configuration in which the inhibition coefficient was at its lowest level for both PqsE and LasR.
After analyzing the growth of P. aeruginosa biofilms near NSAIDs by using Tobramycin and measuring the absorption of crystal violet in ethanol, as a measure of composed biofilm, The findings showed a lower rate of biofilm formation by bacteria treated with NSAIDS and Tombramycin. [P1]
Based on the constructional similarities in some NSAIDs, like Piroxicam, Meloxicam and Homoserine lactones (AHLS), these drugs can be used as quorum-sensing system inhibitors to inhibit LasR and PqsE protein and hence reduce the P. aeruginosa pathogenesis.