Study of Allergen Sequence Databases and Determination of Suitable Databases for Bioinformatics Allergenicity Assessment of Recombinant Proteins

World Allergy Organization has stated that approximately one third of the world population is affected by allergy. Based on regulation of World Health Organization (WHO) allergenicity assessment of recombinant proteins is essential. Allergenicity assessment is facilitated by achieved progress in the determination of 3D structural of allergens and Bioinformatics analysis. Bioinformatics analysis is primary step in allergenicity assessment of recombinant proteins that its result has an important role in weight of evidences. The efficacy of bioinformatics analysis for allergenicity assessment of recombinant proteins depends directly on the nature and content of the databases using for analysis. The aims of this investigation were study and determination of suitable allergen sequence databases for allergenicity assessment of recombinant proteins. Therefore, allergen databases were categorized after comparative study in three groups; A- General, B- molecular analysis and C- motif and epitope. Results indicate that these databases differ in content, organization, and capabilities. In order to get better and more precise results, the bioinformatics assessment must perform with group of allergen sequence databases. Because of number of data and different algorithms about prediction of allergenicity, we recommend FARRP, SDAP, Allergome, ADFS, and Alegpred for bioinformatics allergenicity assessment of recombinant proteins. Also in silico allergenecity assessment of Osmotin was done in this study. The results showed that Osmotin is allergen based on bioinformatic analysis.