Effects of Different Level Zinc Sulfate(ZnSO4) in Diet on the Hematocrit and Hematology Parameters in Gold fish(Carassius auratus)

Inroduction & Mineral materials are the most important part of the fish nutrition subjects diet because they represent a very low quantity in the diet but they have a high effect on fish physiology and metabolism. The aim of this study was investigate the effect of different level of zinc sulfate (ZnSo4) on hematocrit and hematology for assess the effects of this mineral matter on goldfish health.
Experimental diets prepared with four levels(four treatments and each treatment include three repetitions) 0, 25, 75 and 125 mg/kg. Healthy fish with average weight 3.3 ± 0.10 and average length 3.5 ± 0.2 was maintained in 400 liter tanks with temperature 28 ± 1, pH 7.5 ± 0.5 and hardness 286 ± 1.2 and feed for 60 days and end the trial hematocrit and hematology parameter was measured.
Result of analysis the hematological parameter of fish feed with different levels of zinc showed that white blood cells (WBC), mean corpuscular volume (MCV), mean cell hemoglobin (MCH) and mean cell hemoglobin concentration (MCHC) in our experimental treatments did not show a significant difference (p≥0.05) but in red blood cells (RBC), hematocrit (Ht) and hemoglobin (Hb) there was significant difference between treatments (p≤0.05). Red blood cell count, hematocrit and hemoglobin in zinc sulfate treatments (treatments 2, 3 and 4) had higher values and the highest value was in group 4 (150 mg kg zinc sulfate), which there was significant difference between 2 and 3 treatments also had a significant difference with control group.
The results shows that zinc in the diet stimulate the immune system and can have positive effects on the health and hematological factors of goldfish.