Response of Common Bean proteome to Two-Spotted Spider Mite Tetranychus urticae Koch using Proteomics Techniques

Two-spotted spider mite, Tetranychus urticae Koch, is one of the most important pests of Common bean, which causes severe damage on the plants. The purpose of the current study, was determining the bean proteome response to presence and nonpresence of T. urticae using Two-dimensional gel electrophoresis. Bradford method was used to determine the amount of proteins. The results showed that under a nutritional stress, total amount of protein was slightly reduced in comparison with the control. 266 protein spots were identified from bean leaf samples painted by Coomassie Brilliant BlueR-250. Two-dimensional electrophoresis of proteins under a pest nutritional stress showed differences in expression level of leaf protein spots on the control. Overally, eight protein spots lower than the control, had decrease of expression and high expression was showed by four spots. Most of the recognized proteins were related to a direct relationship with system defenses against the stress including HSP, malate dehydrogenase and polyphenol oxidase. These results confirms the response of cells exposed to stress for resistance to the pest feeding. The bands pattern with SDS-PAGE, showed the variation in the expression of bands, in control conditions and the conditions under the treatment with Two-spotted spider mite.