Study of Genetic Diversity of Festuca Arundinacea Based on ISSR Molecular Markers

Fescue is an important forage species growing in wide climates. Genetic variation was estimated for 18 accessions of Festuca arundinacea using 12 ISSR primers in payame Noor university of Kermanshah. In total 87 bands (loci) were obtained.77 bands out of 87 were polymorph in accessions. The mean polymorphism percentage was 89.05 among Fescue accessions.The greatest polymorphism percentage were belonged to IS6, IS9, IS11, IS12 and IS16. Polymorphic information content (PIC) average and Marker index (MI) mean were 0.33 and 2.2 respectively. The highest MI belonged to IS10, IS2, IS6 and IS12. The IS10 and IS6 had the most polymorphism. The greatest similarity belonged to accession 16 (kamiaran)-17 (Irland) and the least similarity belonged to accession1 (Bane)-12(Brojen). Cluster analysis and principal coordinate analysis classified these genotyped to four groups; the accessions of the first group had the highest genetic distance with the accessions of the group 3, based on ISSR markers. These results confirmed by molecular variance analysis as well.