Effect of Iron oxide nanoparticles and magnetic field on neurogenesis and Nestin gene expression after ischemic reperfusion in rat

Ischemic stroke is a common cause of adult disability and death worldwide that leads to damage in neuronal networks and neurovascular units and ceasation of brain functions. In this study the effect of iron oxide nanoparticles and magnetic field on neurogenesis after ischemic reperfusion (IR) in rat model was evaluated.
In this experimental study 50 male Wistar rats weighing 220-250 g were randomly divided into five groups of 10 rats each: including control, sham (ischemic reperfusion model), IR iron oxide nanoparticles (10mg/kg), IR馨❺� field (1 Tesla, 20 min in 4 days), and IR iron oxide nanoparticles and magnetic field groups. Injections were performed intraperitoneally. Neurogenesis was evaluated in hippocampus of 5 groups after 4 days by H&E staining method. Nestin gene expression was quantitatively investigated in 5 groups by Real-time PCR.
In this study it was revealed that the iron oxide nanoparticles as well as the magnetic field increase the neurogenesis rate after ischemic reperfusion during 4 days (p Our result suggests iron oxide nanoparticles as well as magnetic field separately can be two effective methods in ischemia therapy.