Study of the CuO nanoparticles interaction with human serum albumin using fluorescence technique

In this study, interaction between human serum albumin and CuO nanoparticle (NP) at different concentrations was studied using fluorescence method under the simulative physiological condition. The results showed the intrinsic fluorescence reduced in the presence of the NP as the manner increase NP concentration in media. According to the results of quenching rate constant (Kq), it deduced that the interaction take place through static mechanism. Thermodynamic parameters including enthalpy and entropy were calculated as -18.06 (KJ mol-1) and 0.0195 (KJ mol-1 K) respectively, which shows the importance of electrostatic interaction in HAS and NP interaction. Affinity of this particle to HAS deduced as the negative sign of free energy (∆G°) that shows the interaction is exergonic process. Evaluation of ANS fluorescence intensity indicated that hydrophobicity of the surface protein increased in the presence of the NP. Based on the results it established, the protein structure chnaged in the presence of NP which may be affected its function.