Investigating the effect of catechin hydrate on viability, proliferation, and biochemistry of rat bone marrow mesenchymal stem cells in presence of boron as a micronutrient

Aim: Investigating the effect of catechin hydrate (CH) and boric acid (BA) on rat bone marrow mesenchymal stem cells (MSCs).
material and MSCs were treated with culture media containing CH, then with the help of trypan blue the viability was investigated at 12, 24 and 36hours. 400 and 3200µM of CH along with 6ng/ml of BA and 36hours were selected for further study. Proliferation based on colony forming assay (CFA) and population doubling number (PDN), morphology, level of sodium, potassium and calcium and activity of LDH,ALP,AST,ALT were analyzed. Malondialdehyde (MDA), total antioxidant and activity of SOD and CAT were measured too.
only 3200µM at 12hours and from 400 to 6400µM at 24 and 36 hours, the CH caused significant reduction in viability, proliferation, nuclei diameter and cytoplasm area. Treatment with CH caused increase in activity of LDH,ALP,AST,ALT and increased in FRAP as well as reduction of MDA and sodium, potassium level. BA did not show any effect on viability, morphology and PDN but caused reduction of CFA and activity of LDH,AST and ALT. BA also caused elevation of ALP activity and level of calcium, sodium, potassium as well as MDA level and activity of CAT and SOD. Co-treatment compensated the viability, proliferation, morphological changes, metabolic enzyme activity variation and level of electrolyte to some extent. On the other hand, co-treatment showed, CH ameliorated the oxidative stress induced by BA.
since boron ameliorated the CH toxicity, along with tea we may consume dry grapes and dates.