Evaluation of SO6 protein in Saponaria officinalis L. callus

Soapwort (Saponaria officinalis L.) is a native plant to central and southern Europe. It is an important and rare plant species in Iran. The species has numerous applications in medicinal, agricultural and health fields. Saponins is one of the bioactive compounds in the species that is used in chemotherapy. Tissue culture is one of the ways of proliferation of the species that is used for production of active ingredients. Saponin has ten isoforms that are different to inhibition of translating ribosomes and cytotoxicity. SO6 is one of the isoforms of saponins. Presence of SO6 protein in soapwort callus was studied through plant tissue culture and different concentrations of growth regulators and environmental factors. After achieving success in tissue culture, protein was extracted from its callus and presence of SO6 protein was confirmed by western blot analysis and measured by ELISA. Results showed that seed germination was possible through using appropriate treatment and soapwort root callus had the highest amount of SO6 protein.