Analysis of Genetic Structure of Cold Tolerance in Iranian Rice Recombinant Inbred Lines

Rice is one the most important crops in Iran and worldwide. Abiotic stresses including cold, restrict rice production. In order to saturation of linkage map in recombinant lines population caused by Sepidroud × Anbarbou crosses, an experiment was conducted using 96 recombinant lines and 40 ISSR markers at Gonbad Kavous University. 96 Recombinant lines were plant under hydroponic conditions for mapping of traits related to cold stress. Shoot weight, root weight, biomass, genetic score, leaf area, shoot length, root length and root thickness were recorded. Linkage map covered 1709.29 cM of rice genome. Six QTLs mapped in cold stress. qCLA-5 had the highest effects on leaf area with LOD=3.206 and explained 14.3 percent of phenotypic variation. The results of present work can be used for improvement of cold tolerance in rice seedlings after determining marker validation.