Anatomical structure of Medicago sativa L. and assessment of medicarpin in different stages of development

Medicago sativa L is from Fabaceae family that has several flavonoid compound in roots and shoots. Medicarpin is a dominant isoflvonoid that induces in nodul of root in different stage of developmentl. In this study, PAL (phenyl alanine amonialyase) activity was assayed using spectrophotometer. In this rse arc the concentration of medicarpin evaluete by HPLC (high performance liquid chromatography) were studied in different stages of development. The anatomical structure of leaves, stem, root and nodules were studied in transversal section by optical microscope. Different tissue such as epiderme, pharanshime, vascular systeme includes xylem and pheloem, were studied, measured and which are shown in different tabels.The results indicated that activity of PAL enzyme are altered during the plant development stage and changes of medicarpin content were correlated to the activity of PAL enzyme.The highest level of production of medicapin and activity of PAL enzyme have been observed during of growth in root.